News
Two New Heisenberg-Fellowships
Tobias Hermle and Peter Walentek from our department receive funding from the DFG
26.01.2024 14:06
Medical thesis training program
All of our medical students start their medical thesis with a intensive 8 day seminar with theoretical and hands-on bench-side teaching.
| Day 1: | Safety – Introduction – DNA Amplification, expression of genes |
| 8.00 – 9.30 | Safety – Mr. Jenne (Arbeitssicherheit) |
| 9.30 – 10.00 | Introduction
DNA amplification, expression of genes (Dr. Soeren Lienkamp. Dr. Alexis Hofherr, Frau Antje Thien, Frau Alena Ley, Frau Alexandra Schwierzok) |
| 10.00-12.00 | PCR, RT-PCR, generation of RNA, in vivo protein expression, model systems (C. elegans, Xenopus) |
| 13.00-17.00 | Generation of RNA, C. elegans, Xenopus model systems |
| Day 2: | Histology |
| 8:00-10:00 | Detection of antigens in tissues |
| 10:00-17:00 | In situ hybridization tissue section, embedding, staining procedures |
| Day 3: | Microscopy |
| 8:00-10.00 | Introduction to light, fluorescence, widefield and confocal microscopy |
| 10:00-12:00 | Immunoflourescence Staining |
| 13:00-17:00 | Hands on widefield and confocal microscope |
| Day 4: | Manipulation of DNA |
| 8.00-10.00 | DNA, cloning, vectors, plasmid amplification, expression of genes in E. coli |
| 10.00-17.00 | Practical exercise: subcloning, transformation, Mini- and Maxi-Preps, generation of recombinant proteins in bacteria |
| Day 5: | Cell culture |
| 8.00-10.00 | Principles of keeping cells alive in culture, mycoplasm etc. |
| 10.00-17.00 | Cell culture, split 293T cells for transfections |
| Day 6: | Transfections & Transformation |
| 8:00-10.00 | Genetic manipulation of mammalian cells (transfection, transduction, retroviral vectors) |
| 10:00-17.00 | Transfection of gfp, transfection of cells for Western blot |
| Day 7: | Western Blot |
| 8:00-10.00 | Detection of expressed proteins (Western blot, 1D-/2D gels, MS) |
| 10:00-17:00 | Cell lysis, Western blotting |
| Day 8: | Bioinformatics |
| 9:30-15:00 | Webtools, design of PCR primer, sequencing, labbase (Practical exercises) |
